CRISPR Cas9 Gene Editing with FluidFM®
A vision of CRISPR gene editing with FluidFM
To access the nucleus and alter the genetic code, CRISPR complexes require an effective delivery method.
In the literature, multiple gene editing techniques exist. All those methods present a common challenge: find the right cell transfection method to access the nucleus and alter the genome while keeping the cell alive. Despite being the best approach for research areas where multiple simultaneous edits are required within the same cell line or organism, CRISPR gene editing still encounters limitations due to low HDR efficiency and high off target. Nowadays, the FluidFM technology offers an unique in vitro solution to improve the efficiency and applicability of CRISPR across a variety of cell types and for cell line development.
Applications of CRISPR genome editing with FluidFM®
Case study: Multiplex CRISPR editing and monoclonal cell line development
FluidFM nano-injection overcomes delivery limitations of current CRISPR gene editing methods, accelerates cell line development cycles, and is poised to significantly broaden multiplexing capabilities. Pharmaceutical and biological research as well as biologics manufacturing rely on genetically modified cell lines with genes that have been modified to induce the desired phenotype. With the discovery and development of gene editing technologies like CRISPR, the potential of doing multi-loci edits has received much interest but has proven to be a tedious and long process. In the following, we demonstrate the generation of a monoclonal multiple Knock-Out cell line in less than three weeks with the help of the FluidFM technology.
 Jinek, Martin, et al. "A programmable dual-RNA–guided DNA endonuclease in adaptive bacterial immunity." science 337.6096 (2012): 816-821.
 Li, Hongyi, et al. "Applications of genome editing technology in the targeted therapy of human diseases: mechanisms, advances and prospects." Signal transduction and targeted therapy 5.1 (2020): 1-23.