FluidFM versatility:

Unlock original research at the nano-
and micro-scale

Boost collaboration. For service-based core facilities.

3+ FluidFM Core Methods

FluidFM technology enables the below three methods that are the key to many different applications.

  Precise dispensing of liquids

   Intra-cellular injection & extraction

  Pick & place at the micro scale

8+ Applications

Besides the main applications of gene engineering, drug assays and cell isolation, researchers have explored and proved tens of novel FluidFM applications in the fields of material science, life science and biophysics. 


70+ Publications

Since its invention in 2009, FluidFM facilitates cutting-edge research results in life sciences, biophysics and mechanobiology. Explore the 70+ publications.

Explore what can be done with the FluidFM BIO Series:  


  Precise dispensing of liquids. In liquid and dry environments.


  • 2.5D printing: Spotting, lines and custom patterns

  • From µm to cm in scale

  • For any viscosities from water to honey

  • Local dispensing of chemicals and other particles

Odoo - Sample 3 for three columns

Spotting

Precisely deposit thousand spots of any solution. Ideal for developing and testing protein-based assays. 


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Odoo - Sample 3 for three columns

Line-printing

Print lines with widths from tens of micrometers down to hundreds of nanometers.



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Odoo - Sample 2 for three columns

Nano-printing of custom patterns

Print custom patterns from file.

Image: Nanolithography of DAPI on DNA coated glass surface.

Learn more

Odoo - Sample 2 for three columns

Virus deposition

Place virions one-by-one with pin-point accuracy onto single cells.

Learn more

  Intra-cellular injection and extraction.

 
 


Through the microfluidic channel inside a FluidFM probe, solutions can be dispensed or aspirated through a sub-micrometer aperture at the tip of a cantilever. Perform nano-injection experiments and measure the delivered volume with femtoliter precision or extract content directly form specific cells in a culture.

Odoo - Sample 3 for three columns

Single cell CRISPR delivery

A solution to CRISPR's delivery challenges: Place your complexes directly where they are required, in the nucleus. Direct intra-nuclear CRISPR delivery and bottom-up cell line development. Get a multiple knock-out clone in two weeks, with one instrument.

Odoo - Sample 3 for three columns

Single cell drug assays

Deliver compounds directly into a cell and quantify the injected volume with femtoliter precision. A suitable method for any compound and easy to use.


Odoo - Sample 1 for three columns

Single cell biopsy

Extract the content of individual cells directly in their native environment while preserving the cellular context and without affecting cell viability. The procedure is non-destructive and therefore makes it possible to perform cell biopsies from the same single cell over different time points.

Image: Nano-extraction of 1110 fL from a GFP-Hela cell cytoplasm.

Publications

Injection:

Extraction:

  Pick & place at the micro scale.


Reversibly immobilize cells and other objects by gentle application of suction force. A gentle negative pressure through the microfluidic channel of a FluidFM probe allows to pick up and hold a cell. Perform cell isolation or adhesion measurements.

Select a specific cell directly from a culture by mouse click. For suspended and adherent cells.

Odoo - Sample 3 for three columns

Cell isolation

Choose the cell you want – place it where you want.

  • Create in-vitro neuron networks

  • Single cell co-culture for intra-cellular communication

  • Rare cells sorting

  • Gateway to single cell omics

  • Generate monoclonal cell cultures

Odoo - Sample 2 for three columns

Direct cell adhesion assays

Easily measure forces. 

  • From confluent layers

  • Single cells

  • 100s of cells a day, compared to tens reached by conventional means 

Learn more

The right probe for each application

Odoo - Sample 2 for three columns

FluidFM nanosyringe

The sharp apex and the 600 nm aperture at the side of the pyramidal tip enable gentle probe insertion into a cell. Applying over- or under-pressure, you can inject or extract into or from the intra-cellular environment.


Odoo - Sample 1 for three columns

FluidFM nanopipette

The FluidFM nanopipette features a 300 nm aperture at the top of the pyramidal tip. It is ideal for the precise dispensing of liquids for 2.5D patterning applications.



Odoo - Sample 2 for three columns

FluidFM micropipette

It exists with 2, 4 and 8 µm apertures, ideal to reversibly immobilize objects of different sizes by suction force.


Monitor what happens

  • Integrated inverted microscope with 10, 20 and 40 x objectives by Olympus

  • Bright field and fluorescence imaging

  • Multipoint timelapse

Odoo • Text and Image

We are here for your success

A team of scientists is dedicated to help you during the design and execution of FluidFM experiments.


Discuss your experiment with our experts

        References

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