Injection into suspension cells

Direct intra-cellular delivery.

Researchers in immunology, hematology, CAR-T therapy projects, or in biologics production often have to work with non-adherent cells. These cells, like B- or T-cells, are known to be very difficult-to-transfect. In the new era of CRISPR gene editing, finding a tool that can efficiently, gently and easily deliver genetic material – or any kind of molecules – into suspension cells is particularly important for researchers in these fields.

FluidFM offers a unique approach to efficiently deliver by nano-injection any kind of soluble compound, directly into any adherent or suspension cells.

 
 


Video: Real-time video of injection of Lucifer Yellow dye into Jurkat cells. The video shows first the injection procedure in bright field microscopy and then the fluorescent channel to monitor the success rate of injection.

Injection into Jurkat cells

Jurkat cells (Human leukaemic T cell lymphoma) are a non-adherent cell line widely used in research as a model to study T-cell leukemia, T cell signaling or how T cells are infected by Human Immunodeficiency Virus. FluidFM offers a unique tool to researchers in these fields and others working with cells suspension to manipulate the genome or test drugs via nano-injection.

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

Jurkat cells after injection of Lucifer Yellow Dye. Bright-field image.

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

Jurkat cells after injection of Lucifer Yellow Dye. Fluorescence image.

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

Injected Jurkat cell isolated into a separate well plate. Inset shows the fluorescent image of the isolated Jurkat cell.​

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

Injected and isolated Jurkat cell expanded into a monoclonal suspension culture. Bright-field image shows a portion of the culture 7 days after isolation.

Inject, isolate and expand


As for adherent cell lines, FluidFM nano-injection into cells from a suspension culture preserves cellular viability. A single injected cell can be isolated with FluidFM pick & place into a separate well and expanded into a monoclonal culture.

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

CHO cells in suspension, deposited on an anti-fouling coating.

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

CHO cell injected with LY marker.

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

Injected CHO cell isolated into a separate wellplate.

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

CHO cell expanding into a colony on day 3.

Discover the applications

Odoo - Sample 1 for three columns

Single cell CRISPR delivery

Direct intra-nuclear CRISPR delivery and bottom-up cell line development. 
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Odoo - Sample 2 for three columns

Single cell drug assays

Deliver compounds directly into the cell. A suitable delivery method for any compound. More>