Through cell- and nuclear- membranes
No issues due to compounds selective permeability
No need for vectors / chemical modification to pass through the cellular membrane
Target the nucleus or cytoplasm
Deliver compounds directly into the cell.
A suitable delivery method for any compound.
Direct delivery
Bypass membrane impermeability and bring any compound into the intracellular environment.
Easy to use
Intuitive user interface and automated injection process. Keep your focus on the science.
Autonomous delivery
Patented autonomous injection method
Gentle procedure guarantees high cell viability
High reproducibility
Patented autonomous injection method
Gentle procedure guarantees high cell viability
High reproducibility
Deliver any substance
Choose what you inject
Know how much you injected
Only 1 µl of the substance is required to inject 1000's of cells
Choose what you inject
Know how much you injected
Only 1 µl of the substance is required to inject 1000's of cells
Easy handling
System ready in 10 minutes
Full point&click software support
Cell viability for injected HeLa and CHO cells.
> 95% viability
The cellular membrane self-heals upon withdrawal of the FluidFM nanosyringe leading to > 95% viability.
Simplify & accelerate early drug development
Study a drug effect without complications regarding its delivery.
- Decouple drug-effects from side-effects caused by the delivery method.
- Check the effect of your drug before optimizing its delivery.
- Overcome the problem of drug membrane permeability during early drug development.
Know the injected volume for each single cell
The amount of injected drug can be measured with femtoliter precision for each single cell, when co-injected with a fluorescent marker. The volume measurement tool guides you step-by-step towards a self-generated report.
What is the actual delivered volume? Measuring the injected volume per cell allows to precisely know the real drug uptake, thus accounting for cell heterogeneity.
Get dose-response curves at single cell level. Infer on drug properties and their effect at a single cell level.
CHO cells injected with Lucifer Yellow.
Volume quantification for hepatocytes injected with Lucifer Yellow.
Compatible with virtually any compound & cell line
Mouse primary neurons injected with Lucifer yellow and GFP plasmids. Courtesy of Jinan University, Guangzhou, China.
Compounds
Nanoparticles
Lipids
Exogenous proteins
Nucleic acids
And many more, with viscosities from water to honey
Cell lines
Immortalized cell lines
Hard-to-transfect cells
Primary cells
And many more adherent or suspension cell lines
Observe & follow the response
Targeted cells can be long-term imaged to investigate phenomena such us:
- Drug clearance
- Intracellular drug aggregation
- Drug subcellular localization
- Compound toxicity
Protein degradation time-lapse over 5 hours after FluidFM nano-injection.
Selected publications:
Selected publications showing a variety of substances introduced into single cells with FluidFM:
O. Guillaume-Gentil, E. Potthoff, et al. Force-controlled fluidic injection into single cell nuclei. Small, 2013.
A. Meister, M. Gabi, et al. FluidFM: Combining atomic force microscopy and nanofluidics in an universal liquid delivery system for single cell applications and beyond. Nano Letters, 2009.
Principle: FluidFM nano-injection
Through the microfluidic channel inside a FluidFM probe, drug solutions can be dispensed through a sub-micrometer aperture at the tip of a cantilever. The specific probe used in this case is our FluidFM nanosyringe which enables injection into single cells without compromising cell viability. The very sharp apex and the around 600 nm aperture at the front side of the pyramidal tip enable gentle injection and allow working with compounds with varying molecular weights and densities.
FluidFM nanosyringe.
System: Cutting-edge
Designed for pharmacologists and molecular biologists, the FluidFM system is highly automated, easy to use and features a fully enclosing incubator. The temperature-controlled incubator is custom-designed and includes a HEPA filter system. A CO2 control add-on is available upon request. Benefit from simple preparation as well as extensive automation and data management.
Left: FluidFM system without incubator. Right: Custom-designed incubator.
Software: User-friendly & intuitive
Using the high definition image of the cells on the screen, chose the cells of interest according to their phenotype. Select hundreds of cells in a few minutes under visual control.
Follow the in-software suggested instructions to optimally deliver compounds into a cell.
Apply recommended injection settings from our protocols database.
Easily tailor injection parameters to your cells by changing just a few parameters (injection pressure, injection time, exerted force and approach speed).
Finally, step back and let the system autonomously deliver the drug.
After delivery, instruct the system to take the required images with your favorite settings, with a few clicks. All relevant data is stored together with the cell's coordinates.
Simply select the images of the modified cells and follow the on-screen step-by-step instructions to obtain the real injected volume.
Find the injected or chosen cells at any time, and even repeat injection into a same cell.
Left: Cell selection. Middle: Software workflow. Right: Integrated volume calculation tool.
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Direct delivery and precise volume calculation
Get to know FluidFM as a tool for single cell research.
Understand how FluidFM nano-injection works and how it empowers single cell research.
Learn how to measure the volume injected into each single cell and which applications can benefit from it.
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