Through cell- and nuclear- membranes
No issues due to compounds selective permeability
No need for vectors / chemical modification to pass through the cellular membrane
Target the nucleus or cytoplasm
Bypass membrane impermeability and bring any compound into the intracellular environment.
Intuitive user interface and automated injection process. Keep your focus on the science.
No issues due to compounds selective permeability
No need for vectors / chemical modification to pass through the cellular membrane
Target the nucleus or cytoplasm
Patented autonomous injection method
Gentle procedure guarantees high cell viability
High reproducibility
Choose what you inject
Know how much you injected
Only 1 µl of the substance is required to inject 1000's of cells
System ready in 10 minutes
Full point&click software support
Cell viability for injected HeLa and CHO cells.
The cellular membrane self-heals upon withdrawal of the FluidFM nanosyringe leading to > 95% viability.
Simplify & accelerate early drug development
- Decouple drug-effects from side-effects caused by the delivery method.
- Check the effect of your drug before optimizing its delivery.
- Overcome the problem of drug membrane permeability during early drug development.
The amount of injected drug can be measured with femtoliter precision for each single cell, when co-injected with a fluorescent marker. The volume measurement tool guides you step-by-step towards a self-generated report.
What is the actual delivered volume? Measuring the injected volume per cell allows to precisely know the real drug uptake, thus accounting for cell heterogeneity.
Get dose-response curves at single cell level. Infer on drug properties and their effect at a single cell level.
CHO cells injected with Lucifer Yellow.
Volume quantification for hepatocytes injected with Lucifer Yellow.
Mouse primary neurons injected with Lucifer yellow and GFP plasmids. Courtesy of Jinan University, Guangzhou, China.
Compounds
Nanoparticles
Lipids
Exogenous proteins
Nucleic acids
And many more, with viscosities from water to honey
Cell lines
Immortalized cell lines
Hard-to-transfect cells
Primary cells
And many more adherent or suspension cell lines
Protein degradation time-lapse over 5 hours after FluidFM nano-injection.
Selected publications:
Selected publications showing a variety of substances introduced into single cells with FluidFM:
O. Guillaume-Gentil, E. Potthoff, et al. Force-controlled fluidic injection into single cell nuclei. Small, 2013.
A. Meister, M. Gabi, et al. FluidFM: Combining atomic force microscopy and nanofluidics in an universal liquid delivery system for single cell applications and beyond. Nano Letters, 2009.
Get to know FluidFM as a tool for single cell research.
Understand how FluidFM nano-injection works and how it empowers single cell research.
Learn how to measure the volume injected into each single cell and which applications can benefit from it.
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