Abstract: In this talk, I will share my research on Live-seq, a transformative single-cell transcriptomics approach that does not require cell isolation and lysis. Live-seq uses FluidFM Nanosyringes to retrieve cytosolic picoliter biopsies from living cells, which are then analyzed with a highly-sensitive, ultra-low input RNA-seq protocol. The resulting gene expression profiles are faithful representations of intact cell transcriptomes, while the sampled cells remain alive and functional in their original microenvironment. Live-seq makes it therefore possible to perform spatially-resolved longitudinal profiling and phenotyping of individual cells, unlocking exciting opportunities for investigating cellular dynamics and cell-cell communication.