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Keynote Speaker: Live-seq: a FluidFM-based single-cell transcriptomics approach to study cellular dynamics and communication - Session Live-seq & Biopsies
Dr. Orane Guillaume-GentilDone
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Round Table - Live-seq & Biopsies
Done
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Characterization of Mechanotransduction-induced changes in cell identity of PDAC in response to Nanotopography - Session Live-seq & Biopsies
Pr. Dr. Carmelo FerraiDone
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Closing note - FluidFM User Conference 2023
Done
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Coffee Break
Done
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Welcome - Day 2
Done
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Engineering Endosymbiotic Growth of E. coli in Mammalian Cells - Session Genome Engineering
Chantal ErnstDone
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Welcome Note - FluidFM User Conference 2023
Done
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Apero & Posters
Done
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Transient Changes in Stem Cells Induced by Electrical Stimulation - Session Mechanobiology
Dr. Amy GelmiDone
Abstract
Single-cell RNA sequencing has revolutionised our understanding of cellular heterogeneity. However, conventional methods involve cell lysis and lack the ability to directly investigate dynamic trajectories underlying cellular state transitions, often relying on inference. Recently, Live-seq pioneered the temporal mRNA analysis of the same cell over two days via longitudinal cytoplasmic extraction using fluidic force microscopy. Here, we present a nanobiopsy platform that enables simultaneous injections and cytoplasmic sampling from an individual cell and its progeny without killing it. The technique is based on scanning ion conductance microscopy (SICM) and employs a double-barrel nanopipette to introduce exogenous molecules and longitudinally profile the transcriptome of individual glioblastoma (GBM) brain tumour cells in vitro over 72hrs, with and without standard treatment, including chemotherapy and radiotherapy. Our results suggest that treatment either induces or selects for more transcriptionally stable cells. We envision the nanobiopsy will contribute to the new emerging field that will transform standard single-cell transcriptomics from a static analysis into a dynamic and temporal assay.