single cell EXPERIMENTS REINVENTED.
FluidFM® BOT
A POWERFUL TOOL FOR SINGLE CELL BIOLOGY.
The FluidFM BOT is a revolutionary tool for biologists in particular in cell line, drug development or genetic editing at the single cell level.
The cutting-edge FluidFM BOT system opens the door to single cell experiments that have not been possible with conventional methods. Built around the patented FluidFM hollow probe technology, the highly automated system allows cell manipulation at the single cell level, and this in a non-destructive and cell-context preserving manner.
STATE-OF-THE-ART
EXTENSIVE AUTOMATION
COMPLETE
STAND-ALONE SYSTEM
SIMPLE
INTUITIVE SOFTWARE & WORKFLOW
ARYA
FULL EXPERIMENTAL CONTROL
nano-injection.
The FluidFM BOT is an unmatched tool to introduce a vast variety of materials of your choice into a cell.
Inject a vast variety of compounds such as DNA, RNA and plasmids directly into nuclei or cytoplasms of adherent cells. This is done in a ”gentle”, less stressful manner than with most other delivery methods, resulting in cell viability of ~95%. In addition, it is possible to measure the injected femtoliter volumes (fL) and inject 100 to 300 cells/hour depending on the cell types.
Adherent cell lines already successfully nano-injected into with the FluidFM BOT:
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C2C12
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HEK (Human embryonic kidney cell line)
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Mouse primary hepatocytes
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CHO (Chinese hamster ovary cells)
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MEFs (Mouse embryonic fibroblasts)
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hESCs (Human embryonic stem cells)
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mESCs (Mouse embryonic stem cells)
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Pig neurons
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HeLa (Cervical cancer cell line)
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HepG2 (Human liver cancer cell line)
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U2OS (Human osteosarcoma cell line)
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MCF 7
Would you like to nano-inject into another cell line?
SELECTED PUBLICATIONS
O. Guillaume-Gentil, E. Potthoff, D. Ossola, et al. Force-controlled fluidic injection into single cell nuclei. (2013) Small, 9(11), 1904 — 1907. doi:10.1002/smll.201202276
A. Meister, M. Gabi, P. Behr, et al. FluidFM: Combining atomic force microscopy and nanofluidics in a universal liquid delivery system for single cell applications and beyond. (2009) Nano Letters, 9(6), 2501-2507. doi:10.1021/nl901384x
PRECISE
IN CYTOPLASM OR NUCLEUS
FAST
100+ CELLS / HOUR
GENTLE
~95% CELL VIABILITY
MEASURABLE
FEMTOLITER INJECTED VOLUMES
THE SOLUTION FOR YOUR RESEARCH QUESTIONS.
Injected Volume Estimation
Working with fluorescent compounds, the microfabricated FluidFM nanosyringe serves as a precise reference for calculating the injected volume.
FluidFM Optimizes CRISPR-Cas Delivery
Perform fast nano-injection of CRISPR-Cas complexes, especially into cells difficult to target with conventional CRISPR delivery methods.
FluidFM Enhances Plasmid Transfection
Transfection of plasmids with FluidFM nano-injection enables the delivery of genetic material even into difficult to transfect cells and in a faster and, for the cells, less stressful manner than with conventional methods.
PRECISE
FEMTOLITER SCALE
CUTTING-EDGE
INJECT INTO DIFFICULT TO TRANSFECT CELLS
CELL FRIENDLY
NO TOXIC COMPOUNDS
nano-EXTRACTION & cell ISOLATION.
Amongst many applications, FluidFM nano-extraction and FluidFM cell isolation will critically empower your single cell research. Together with FluidFM nano-injection, these applications make the FluidFM BOT an unrivalled tool in many key research areas.
Nano-extraction
Using the FluidFM nanosyringe, perform gentle biopsies on selected cells. The approach is similar to FluidFM nano-injection but uses negative pressure to extract the cell content. Depending on your interest, target either the nucleus or the cytoplasm of the selected cells. The precise dimensions of the nanosyringe allow you to control the volume of the biopsy. The image above shows the nano-extraction of 1110 fL from a GFP-Hela cell cytoplasm. The procedure is non-destructive and therefore makes it possible to perform cell biopsies from the same single cell over different time points.
Cell isolation
Adherent cells can easily be isolated using the FluidFM micropipette. Selectively shower the cell of interest with trypsin or any other dissociation reagent. Then, apply a gentle negative pressure to harvest the cell with the micropipette. You can then move to another well to isolate the cell by approaching the surface and applying a positive pressure through the microfluidic channel of the micropipette.
CELL BIOPSIES
NON-DESTRUCTIVE
DIVERSE
COMBINE DIFFERENT FluidFM APPLICATIONS
ISOLATION
FROM ADHERENT CELL CULTURE
towards full automation.
Simple preparation and extensive automation and data management combined with our user-friendly and intuitive ARYA software allows any user to inject more than a hundred cells per hour.
On the screen, simply point and click on the cytoplasm or nucleus of the cells you want to target, and press "OK". It is as straightforward as that.
The application-specific workflow within ARYA allows all users to be in full control of their experimental parameters and to optimize them depending of the type of cells that are to be manipulated.
Due to the fully programmable FluidFM nanosyringe exchange and washing features and automatic sample swapping, all experiments are executed in a clean and precise fashion, reducing contamination risks and human error to a minimum, while boosting productivity.
SIMPLE
INTUITIVE SOFTWARE & WORKFLOWS
STRAIGHTFORWARD
POINT & CLICK TO INJECT
HIGH AUTOMATION
HIGHER PRODUCTIVITY
FluidFM BOT SYSTEM.
A high level of automation coupled with intuitive instrument operation have proven to be particularly important for single cell research.
The FluidFM BOT is a complete stand-alone system which includes all essential elements such as a motorized inverted microscope and an anti-vibration table with its own pressure pump assuring stable FluidFM experiments. The system also includes a made-to-measure incubator with a HEPA filter and UV light to minimize contamination.
With the FluidFM BOT comes also a set of FluidFM probes, including the FluidFM nanosyringe. A dedicated barcode reader gives convenient access to the relevant probe characteristics and quality control data. Finally, an optimized computer and a high definition monitor enable smooth operation of the ARYA software and all FluidFM BOT system components.
STATE-OF-THE-ART
EXTENSIVE AUTOMATION
ARYA
FULL EXPERIMENTAL CONTROL
COMPLETE
STAND-ALONE SYSTEM
bio-lab.
easy.
Enjoy simple and intuitive instrument operation via our Award winning ARYA Operator software
fast.
Boost your research productivity with the FluidFM BOT. Enjoy higher experimental throughput without sacrifices.
automated.
Take advantage from an unprecedented high degree of automation for your applications with the FluidFM BOT.