CellEDIT - CRISPR-Edited C2C12 Cell Line

Let us support you with a custom, high-quality engineered C2C12 cell line.

We'll handle the CRISPR engineering of your C2C12 cells, so you focus on your research.


C2C12 is a mouse myoblast cell line which quickly differentiates to form contractile myotubes and produce characteristic muscle proteins.

C2C12 Cell Line Information

Organism

Mouse

Tissue Type

Muscle

Cell Type

Myoblast

C2C12 Cell Line Applications


 Muscle Development and Differentiation: C2C12 cells are myoblast cells that can be readily differentiated into myotubes and are therefore extensively used to study the mechanisms of muscle development, differentiation, and regeneration.

 

 Gene Expression Studies: Researchers utilize C2C12 cells to investigate the regulation of gene expression during muscle cell differentiation, providing insights into muscle-specific genes and transcriptional control mechanisms.

 

 Drug Screening and Development: The cell line is employed in testing the effects of various compounds on muscle cells, aiding in the discovery and development of drugs for muscle-related diseases and conditions.

 

 Muscle Disease Modeling: C2C12 cells are used to model muscle diseases, such as muscular dystrophy, enabling researchers to study disease pathology and potential therapeutic approaches.


 Tissue Engineering and Regenerative Medicine: The cell line is used to develop and test scaffolds and other materials intended for muscle repair and regeneration in regenerative medicine applications.

 

Related Case Studies


Maximize your research potential with CellEDIT’s expertise. A custom CRISPR-edited C2C12 cell line is crucial for studying muscle development and differentiation, as it can differentiate into myoblast and myotubes. It provides valuable insights into gene expression regulation during muscle formation and is widely used in drug screening for muscle-related diseases. Additionally, C2C12 cells are essential for modeling muscle diseases and advancing tissue engineering and regenerative medicine research. With the CellEDIT workflow, we can provide you with custom and monoclonal knockout C2C12 cell lines suited for your project needs, with a 10-week delivery time.

CellEDIT Engineered C2C12 Cell Line - Cytosurge
Efficient on 
Hard-to-transfect cells
CellEDIT Engineered C2C12 Cell Line - Cytosurge
Vector-free 
direct intra-nuclear delivery
CellEDIT Engineered C2C12 Cell Line - Cytosurge
Minimized Off-Target

Immortalized Cells

Find out how 
the CellEDIT workflow enabled the generation of 5 monoclonal HPRT  knockout C2C12 cell lines through direct intra-nuclear injection of only 51 cells.

Hard-to-Transfect Cells

Discover how 
the CellEDIT workflow facilitated the production of 3 monoclonal HPRT1 knockout SK-MES-1 cell lines, which are notoriously hard-to-transfect.

 Available Edits using CellEDIT Engineered Cells


Knockout

Save time in the lab by empowering your research with CellEDIT. Confidently streamline your drug discovery workflow and investigate gene function with effective CRISPR knockouts.


 Find out more

Multiplex Editing

At CellEDIT, we provide you with multiplexed cell lines in 10 weeks. The efficiency and gentleness of our vector-free editing through intra-nuclear delivery, makes it an ideal system to perform multiplex gene editing in one go.


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Schedule Now A Meeting to Discuss Your C2C12 Cell Line Edits

 Related Resources

Media & Downloads

On-Demand CellEDIT's CRISPR Cell Line Engineering Webinar

CellEDIT's Service Brochure

CellEDIT's Application Note n*1 - Introducing the CellEDIT Workflow

CellEDIT's Application Note n*2 - Overcoming the hard-to-transfect cell line hurdle

Open Access Publication featuring CellEDIT:  Antony, Justin S., Anabel Migenda Herranz, Tahereh Mohammadian Gol, Susanne Mailand, Paul Monnier, Jennifer Rottenberger, Alicia Roig‐Merino et al. " Accelerated generation of gene-engineered monoclonal CHO cell lines using FluidFM nanoinjection and CRISPR/Cas9Biotechnology Journal 19, no. 4 (2024): 2300505.

Case Study - Streamlined U2OS Cell Line Modification with the CellEDIT Service Workflow, featuring our CellEDIT Customer: Dr. Kanstantsin Siniuk.

Browse All Engineered Cell Lines