Direct intra-nuclear CRISPR delivery.

Place your complexes directly where they are required :
in the nucleus.

A solution to CRISPR’s delivery challenges.

The FluidFM nanosyringe gets through extracellular matrices, cell membranes and cytoplasmatic elements.

Direct delivery with a simple mouse click.

Automated and reproducible injection process with an intuitive user interface.

 
 

Gene engineering and editing without the limitations of genetic material transport.

 

 

For any cargo

  • siRNA, mRNA, transcription factors

  • CRISPR

  • gRNA, nucleases, DNA template, plasmids

  • Artifical chromosomes

  • ...

Less degradation

  • Direct delivery reduces the exposure of the genetic material to a cell's metabolic processes

  • Less degradation of the delivered genetic material

Less cellular stress

  • With greater transport efficiency, less genetic material is needed

  • Less cytotoxicity, less side effects and generally less cellular stress

For any adherent cell

Higher HDR efficiency

Simultaneous co-location is the key.

For HDR to happen, the Cas9 protein, gRNA and repair template need to be simultaneously co-located.

With the FluidFM nanosyringe, all CRISPR components are delivered simultaneously and at the right concentration into the nucleus.

Odoo • Text and Image

Optimize off-target effects

Precise dosage, less side effects

Enabled by our Volume Calculator. Learn more.

Just the right concentration .  FluidFM helps to effectively minimize off-target effects by avoiding an excess of Cas9 availability.

As the quantity of injected copies can be calculated with the volume measurement tool, dose/response relationships can be investigated and thus off-target effects effectively minimized.

Cas9 proteins are active from the very moment the injection takes place, as they are delivered directly into the nucleus together with the associated gRNAs and repair templates.

Get in contact to minimize off-target effects

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

Cell viability for injected HeLa and CHO cells. 

> 95% viability

The cellular membrane self-heals upon withdrawal of the FluidFM nanosyringe leading to > 95% viability.

Straight-forward multiplex & stack editing

  • Full freedom in designing the injected cocktail: a nano-syringe can be loaded with 100's of different gRNAs. Biology is the limit.

  • Inject different cocktails at different time points: >95% of cells survive the injection procedure.

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

4 serial injections into the same cell. Lucifer Yellow was used to calculate the volume of solution injected.

    Gallery

    Transfected cells with FluidFM

    Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

    Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes.

    Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

    GFP expression in neurons 24h after nano-injection of plasmids into a mouse primary neuron. Courtesy of Jinan University, Guangzhou, China.

    Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

    pEGFP-UHRF1 and pmCherry-TRIM21 (2 plasmids) coinjected into CHO-K1 cells. The nuclear expression of the human UHRF1 protein is observed (3h post injection), as well as the cytoplasmic expression of the human TRIM21 protein.

    Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes with the FluidFM Bio-CRISPR.

    Mouse primary hepatocyte injected with CRISPR-Cas9 RNP complexes .

          Webinar with a live demo:

          FluidFM: a new approach to CRISPR gene editing

          A webinar together with Olympus Life Science and Oxford Global showing you how to overcome one of the biggest challenges in gene editing: direct delivery of reagents into the nucleus.

           

          Odoo • Text and Image



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