Use Cases: Direct intra-nuclear delivery with FluidFM.

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Shorter cell line development times: transfection and selection in few weeks. Monoclonality guaranteed. 


Find out how FluidFM can generate multiple KO clones in two weeks in three simple steps:

1.Injection of multiple gRNAs&Cas9 - A fluorescent marker is co-injected into CHO cells to verify successful delivery. 

  • Multiple gRNAs are simultaneously delivered 

  • Viability: >95%

2.Isolation of the clone candidate - Selected cells are isolated into separate wells.

3.Clone expansion and analysis - Monoclonal colonies are expanded and the DNA sequenced. 50% of the colonies show a mutation in all the targeted loci.

  • Extreme reduction in complexity and development time of modified cell lines.

  • Precise, minimal footprint: Monoclonal cell line generated starting from few nano-injected cells


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Successful transfection of primary neurons with plasmids

With our system, even primary neurons can be transfected successfully and highly efficiently. The FluidFM nanosyringe directly delivers the complexes into primary neurons - gently and autonomously.

Conventional delivery methods applied to neurons are often inefficient, expensive and toxic to the neuron.

Image: GFP expression in neurons 24h after nano-injection of plasmids in a mouse primary neuron. Courtesy of Jinan University Guangzhou, China.


Odoo • Text and Image

Transient expression of target proteins with mRNA

Human Dermal Fibroblast injected with GFP mRNA. 70% of injected cells expressed GFP 24h after injection.

Transient mRNA transfection helps to give fundamental insights on protein functions at a single cell level, such as protein expression and intercellular communication.

mRNA transfection using FluidFM nano-injection results in:

  • Higher transfection efficiency

  • Lower cell toxicity

  • Faster expression

Image: Human dermal fibroblast expressing GFP, 24h after injection.

Transfected cells with FluidFM

Fluorescent CRISPR-Cas9 complexes injected into mouse primary hepatocytes.

Fluorescent CRISPR-Cas9 complexes directly injected into mouse primary hepatocytes.

GFP expression in neurons 24h after nano-injection of plasmids into a mouse primary neuron. Direct nuclear injection with FluidFM.

GFP expression in neurons 24h after nano-injection of plasmids into a mouse primary neuron. Courtesy of Jinan University, Guangzhou, China.

Nuclear and cytoplasmic expression - pEGFP-UHRF1 and pmCherry-TRIM21 (2 plasmids) coinjected into CHO-K1 cells.

pEGFP-UHRF1 and pmCherry-TRIM21 (2 plasmids) coinjected into CHO-K1 cells. The nuclear expression of the human UHRF1 protein is observed (3h post injection), as well as the cytoplasmic expression of the human TRIM21 protein.

Mouse primary hepatocyte injected with CRISPR-Cas9 RNP complexes .

Mouse primary hepatocyte injected with CRISPR-Cas9 RNP complexes.

Webinar with a live demo:

FluidFM: a new approach to CRISPR gene editing

A webinar together with Olympus Life Science and Oxford Global showing you how to overcome one of the biggest challenges in gene editing: direct delivery of CRISPR-Cas complexes into the nucleus.

Selected publications 

Introducing substances, such as genetic vectors, into single cells with FluidFM has been established since several years:


Optimize off-target effects. Perform straight-forward multiplex editing.

Learn more about the advantages of the FluidFM BOT BIO Series.

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