Use Cases: Direct intra-nuclear delivery with FluidFM.
Find out how our FluidFM technology can can help your research.
Generation of multiple KO clones. One instrument, < 3 weeks. Monoclonality guaranteed.
Find out how FluidFM can generate multiple KO clones in < 3 weeks in three simple steps:
Day 1: Injection of multiple gRNAs & Cas9
Multiple gRNAs are simultaneously delivered with the Cas9 into CHO cells. A fluorescent marker is co-injected to verify successful delivery.
Hands-on time: 30 minutes - from the loading of the FluidFM Nanosyringe until the injection of 50 cells is completed
Day 2: Isolation of the clone candidate
Selected cells are isolated into separate wells. Visual proof of single cell deposition ensures monoclonality.
Hands-on time: 30 minutes - for the preparation of the FluidFM Micropipette and the isolation of 12 injected cells expressing the fluorescent marker
As of day 3: Clone expansion and analysis
Of the isolated clones, 90% developed into monoclonal colonies and 50% thereof showed mutations in targeted loci.
Strong reduction in complexity and development time of genetically modified cell lines starting from few nano-injected cells
Successful transfection of primary neurons with plasmids
With our system, even primary neurons can be transfected successfully and highly efficiently. The FluidFM nanosyringe directly delivers the complexes into primary neurons - gently and autonomously.
Conventional delivery methods applied to neurons are often inefficient, expensive and toxic to the neuron.
Image: GFP expression in neurons 24h after nano-injection of plasmids in a mouse primary neuron. Courtesy of Jinan University Guangzhou, China.
Transient expression of target proteins with mRNA
Human Dermal Fibroblast injected with GFP mRNA. 70% of injected cells expressed GFP 24h after injection.
Transient mRNA transfection helps to give fundamental insights on protein functions at a single cell level, such as protein expression and intercellular communication.
mRNA transfection using FluidFM nano-injection results in:
Higher transfection efficiency
Lower cell toxicity
Image: Human dermal fibroblast expressing GFP, 24h after injection.
Transfected cells with FluidFM
Fluorescent CRISPR-Cas9 complexes directly injected into mouse primary hepatocytes.
GFP expression in neurons 24h after nano-injection of plasmids into a mouse primary neuron. Courtesy of Jinan University, Guangzhou, China.
pEGFP-UHRF1 and pmCherry-TRIM21 (2 plasmids) coinjected into CHO-K1 cells. The nuclear expression of the human UHRF1 protein is observed (3h post injection), as well as the cytoplasmic expression of the human TRIM21 protein.
Mouse primary hepatocyte injected with CRISPR-Cas9 RNP complexes.
Webinar with a live demo:
FluidFM: a new approach to CRISPR gene editing
A webinar together with Olympus Life Science and Oxford Global showing you how to overcome one of the biggest challenges in gene editing: direct delivery of CRISPR-Cas complexes into the nucleus.
Introducing substances, such as genetic vectors, into single cells with FluidFM has been established since several years:
O. Guillaume-Gentil, E. Potthoff, et al. Force-controlled fluidic injection into single cell nuclei. Small, 2013.
A. Meister, M. Gabi, et al. FluidFM: Combining atomic force microscopy and nanofluidics in an universal liquid delivery system for single cell applications and beyond. Nano Letters, 2009.
Optimize off-target effects. Perform straight-forward multiplex editing.
Learn more about the advantages of the FluidFM BOT BIO Series.